| Enzyme Activity: | Esterase |
| EC Number: | 3.1.1.73 |
| CAZy Family: | CE1 |
| CAS Number: | 134712-49-5,
224306-54-1,
224306-55-2 |
| Synonyms: | feruloyl esterase; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase |
| Source: | Rumen microorganism |
| Molecular Weight: | 29,000 |
| Concentration: | Supplied at ~ 400 U/mL |
| Expression: | Recombinant from Rumen microorganism |
| Specificity: | Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates. |
| Specific Activity: | ~ 100 U/mg (40oC, pH 6.5 on ethyl ferulate) |
| Unit Definition: | One Unit of feruloyl esterase activity is defined as the amount of enzyme required to release one µmole of ferulic acid per minute from ethyl-ferulate (0.39 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 7 |
| Application examples: | Applications established in biofuels, paper and pulp, food, nutrition, medical and pharmacological industries. |
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